ABSTRACT
OBJECTIVE: To investigate the mechanism of hyperthyroid rats treated by thiamazole tablets by the serum metabonomics technology. METHODS: Hyperthyroid rats were modeled by subcutaneous injection of L-levothyroxine sodium 35 μg · 100 g-1 for 7 d. Then rats of thiamazole-treated group were treated by intragastric administration of thiamazole tablets suspension 21 mg · kg-1 for 21 d. The serum levels of model rats, control rats, thiamazole-treated rats were analyzed by using UPLC-QTOF-MS and UPLC-QTOF-MS was combined with two different modes of positive and negative ions as well as KEGG and HMDB. RESULTS: Compared with normal rats, among hyperthyroid rats oxalacetic acid, dihydroxyacetone phosphate (DHAP), ethyl glucuronide, leukotriene E3, hydroxykynurenine, triglycerides (TG) and lysophospholipids were increased. Meanwhile, diacylglycerol (DG) and phosphatidylinositol (PI) were decreased. The 21 d later, the metabolism levels of oxalacetic acid, dihydroxyacetone phosphate, leukotriene E3, phospholipids and fatty acids in thiamazole-treated rats were close to the normality. CONCLUSION: The results infer that tricarboxylic acid cycle of hyperthyroid rat is disturbed: all of glucose metabolism, lipid metabolism and amino acid metabolism get into disorder. And these disorders are improved certainly by treating thiamazole. Meanwhile, this method provides an available reference for the establishment of the means of diagnosing hyperthyroidism based on metabonomics and the assessment of the therapeutic effect of thiamazole tablets.